ctx i Search Results


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Bone formation was increased in male but not Cav-1−/− female mice. A–C, sections of tibia were stained with hematoxylin/eosin and for TRAP activity. A, representative images of stained sections are shown. BV/TV was quantitated from the tibia sections. B, quantitative analysis using the sections include the following: OC number/bone perimeter (N.Oc/B.Pm); OC surface/bone surface (Oc.S/BS); eroded surface/bone surface (ES/BS). C, OB number/bone perimeter (N.Ob/B.Pm) and OB surface/bone surface (Ob.S/BS) are shown. D, <t>serum</t> <t>CTX-I</t> and PINP levels were measured by ELISA. E, WT and Cav-1−/− mice were intraperitoneally injected with calcein followed by xylenol orange with a 7-day interval. Tibia were isolated at 3 days after final injection for von Kossa stain and confocal microscopy imaging. Quantitative analysis using the sections include the following: BFR/MS, MAR, and MS/BS. Fluorescence labels are indicated by arrows. n = 5 per group. *, p < 0.05; **, p < 0.005 as compared with WT.
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Bone formation was increased in male but not Cav-1−/− female mice. A–C, sections of tibia were stained with hematoxylin/eosin and for TRAP activity. A, representative images of stained sections are shown. BV/TV was quantitated from the tibia sections. B, quantitative analysis using the sections include the following: OC number/bone perimeter (N.Oc/B.Pm); OC surface/bone surface (Oc.S/BS); eroded surface/bone surface (ES/BS). C, OB number/bone perimeter (N.Ob/B.Pm) and OB surface/bone surface (Ob.S/BS) are shown. D, <t>serum</t> <t>CTX-I</t> and PINP levels were measured by ELISA. E, WT and Cav-1−/− mice were intraperitoneally injected with calcein followed by xylenol orange with a 7-day interval. Tibia were isolated at 3 days after final injection for von Kossa stain and confocal microscopy imaging. Quantitative analysis using the sections include the following: BFR/MS, MAR, and MS/BS. Fluorescence labels are indicated by arrows. n = 5 per group. *, p < 0.05; **, p < 0.005 as compared with WT.
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Bone formation was increased in male but not Cav-1−/− female mice. A–C, sections of tibia were stained with hematoxylin/eosin and for TRAP activity. A, representative images of stained sections are shown. BV/TV was quantitated from the tibia sections. B, quantitative analysis using the sections include the following: OC number/bone perimeter (N.Oc/B.Pm); OC surface/bone surface (Oc.S/BS); eroded surface/bone surface (ES/BS). C, OB number/bone perimeter (N.Ob/B.Pm) and OB surface/bone surface (Ob.S/BS) are shown. D, <t>serum</t> <t>CTX-I</t> and PINP levels were measured by ELISA. E, WT and Cav-1−/− mice were intraperitoneally injected with calcein followed by xylenol orange with a 7-day interval. Tibia were isolated at 3 days after final injection for von Kossa stain and confocal microscopy imaging. Quantitative analysis using the sections include the following: BFR/MS, MAR, and MS/BS. Fluorescence labels are indicated by arrows. n = 5 per group. *, p < 0.05; **, p < 0.005 as compared with WT.
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Immunodiagnostic Systems ctx-i ratlaps eia
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Image Search Results


Bone formation was increased in male but not Cav-1−/− female mice. A–C, sections of tibia were stained with hematoxylin/eosin and for TRAP activity. A, representative images of stained sections are shown. BV/TV was quantitated from the tibia sections. B, quantitative analysis using the sections include the following: OC number/bone perimeter (N.Oc/B.Pm); OC surface/bone surface (Oc.S/BS); eroded surface/bone surface (ES/BS). C, OB number/bone perimeter (N.Ob/B.Pm) and OB surface/bone surface (Ob.S/BS) are shown. D, serum CTX-I and PINP levels were measured by ELISA. E, WT and Cav-1−/− mice were intraperitoneally injected with calcein followed by xylenol orange with a 7-day interval. Tibia were isolated at 3 days after final injection for von Kossa stain and confocal microscopy imaging. Quantitative analysis using the sections include the following: BFR/MS, MAR, and MS/BS. Fluorescence labels are indicated by arrows. n = 5 per group. *, p < 0.05; **, p < 0.005 as compared with WT.

Journal: The Journal of Biological Chemistry

Article Title: Caveolin-1 Regulates Osteoclastogenesis and Bone Metabolism in a Sex-dependent Manner *

doi: 10.1074/jbc.M114.598581

Figure Lengend Snippet: Bone formation was increased in male but not Cav-1−/− female mice. A–C, sections of tibia were stained with hematoxylin/eosin and for TRAP activity. A, representative images of stained sections are shown. BV/TV was quantitated from the tibia sections. B, quantitative analysis using the sections include the following: OC number/bone perimeter (N.Oc/B.Pm); OC surface/bone surface (Oc.S/BS); eroded surface/bone surface (ES/BS). C, OB number/bone perimeter (N.Ob/B.Pm) and OB surface/bone surface (Ob.S/BS) are shown. D, serum CTX-I and PINP levels were measured by ELISA. E, WT and Cav-1−/− mice were intraperitoneally injected with calcein followed by xylenol orange with a 7-day interval. Tibia were isolated at 3 days after final injection for von Kossa stain and confocal microscopy imaging. Quantitative analysis using the sections include the following: BFR/MS, MAR, and MS/BS. Fluorescence labels are indicated by arrows. n = 5 per group. *, p < 0.05; **, p < 0.005 as compared with WT.

Article Snippet: Determination of C-terminal Peptide of Type I Collagen (CTX-I) and N-terminal Propeptide of Type I Collagen (PINP) CTX-I levels in 8-week-old WT and Cav-1 −/− mice serum were measured using CTX-I and PINP ELISA kits (Immunodiagnostic Systems, Boldon, UK) following the manufacturer's instruction.

Techniques: Staining, Activity Assay, Enzyme-linked Immunosorbent Assay, Injection, Isolation, Confocal Microscopy, Imaging, Fluorescence

Key resources table

Journal: Journal of Bone and Mineral Research

Article Title: Temporal patterns of osteoclast formation and activity following withdrawal of RANKL inhibition

doi: 10.1093/jbmr/zjae023

Figure Lengend Snippet: Key resources table

Article Snippet: Serum RatLaps (CTX-I) EIA , Abacus ALS Pty Ltd , Cat#: IDSAC06F1.

Techniques: Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Gene Expression, cDNA Synthesis, Software, Real-time Polymerase Chain Reaction